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type culture collection tib 202 huvec american type culture collection pcs 100 013 hpde6 c7 anwei sci cell center aw ch0777 kpc 0116 fuscc  (ATCC)


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    ATCC type culture collection tib 202 huvec american type culture collection pcs 100 013 hpde6 c7 anwei sci cell center aw ch0777 kpc 0116 fuscc
    Type Culture Collection Tib 202 Huvec American Type Culture Collection Pcs 100 013 Hpde6 C7 Anwei Sci Cell Center Aw Ch0777 Kpc 0116 Fuscc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcs+100+013/Primary+Umbilical+Vein+Endothelial+Cells%3B+Normal%2C+Human%2C+Pooled/pm42276047-732-169-174
    Average 99 stars, based on 1334 article reviews
    type culture collection tib 202 huvec american type culture collection pcs 100 013 hpde6 c7 anwei sci cell center aw ch0777 kpc 0116 fuscc - by Bioz Stars, 2026-09
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    Cell Culture:

    Article Title: Hypomethylation in MTNR1B : a novel epigenetic marker for atherosclerosis profiling using stenosis radiophenotype and blood inflammatory cells
    Article Snippet: DNA from the buffy coats and individual inflammatory cell types was extracted using a kit (DNeasy Blood & Tissue Kit, Qiagen, Germany) and stored at − 20 °C until further use. .. For wild-type endothelial cells, we cultured five human umbilical vein endothelial cell (HUVEC) lines (cat. no. C-005-5C, lot no. 1774129, Gibco, USA; cat. no. C-015-5C, lot no. 1391153, Gibco; cat. no. PCS-100-010, lot no. 70006858, American Type Culture Collection [ATCC]; cat. no. PCS-100-010, lot no. 70008844, ATCC; cat. no. PCS-100-013, lot no. 80616172, ATCC) and two human aortic endothelial cell (HAEC) lines (cat. no. PCS-100-011, lot. no. 64389694 and 70008309, ATCC). .. HUVECs and HAECs were cultured in M199 growth media (cat. no. 31100035, Gibco) containing less than 20% fetal bovine serum (cat. no. 12483-020, Gibco), 2% human serum, 2 mmol/L L-glutamine (cat. no. 25030-081, Gibco), and 50 μg/mL endothelial cell growth supplement (cat. no. 356006, BD Biosciences, USA).

    Article Title: Mast cell extracellular granules are bioactive condensates assembled by heparin and polyamine.
    Article Snippet: .. HUVECs (American Type Culture Collection (ATCC), PCS-100-013) were cultured in vascular cell basal medium supplemented with endothelial cell growth kit BBE (ATCC) and incubated at 37 °C with 5% CO2. .. Inhibitors, namely APCHA 79 (0.5 mM; TCI chemicals), reserpine80 (10 μM; Cayman Chemical) or Baf-A1 (ref. 81) (20 nM; Cayman Chemical), were added to mast cell culture 3 days before subsequent experiments.

    Article Title: Heme drives cardiac endothelial senescence in sepsis via STING activation
    Article Snippet: .. Human umbilical vein endothelial cells (HUVECs) were obtained from the American Type Culture Collection (ATCC, PCS-100-013) and cultured in Vascular Cell Basal Medium (ATCC, PCS-100-030) supplemented with the Endothelial Cell Growth Kit–VEGF (ATCC, PCS-100-041). ..

    Article Title: MSC-derived exosomal circMYO9B accelerates diabetic wound healing by promoting angiogenesis through the hnRNPU/CBL/KDM1A/VEGFA axis
    Article Snippet: .. Human umbilical vein endothelial cells (HUVECs) obtained from American Type Culture Collection (RRID: PCS-100-013) were authenticated by STR profiling and tested for mycoplasma contamination, and then cultured in Medium 200 (Gibco), Three doses of glucose were used to treat HUVECs including 5.5 mM (normal glucose, NG), 15 and 30 mM (high glucose, HG). ..

    Article Title: STEAP3 alleviates inflammation and fibrosis via iron metabolism in ischemia/reperfusion-associated lung injury.
    Article Snippet: .. Human Umbilical Vein Endothelial Cells (HUVECs; American Type Culture Collection [ATCC], PCS-100-013) were cultured in Dulbecco's Modified Eagle's Medium (DMEM) with 10 % FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 ◦C with 5 % CO2. .. THP-1 cells (ATCC, TIB-202) were cultured in RPMI 1640 medium with 10 % FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 ◦C with 5 % CO2.

    Article Title: Mast cell extracellular granules are bioactive condensates assembled by heparin and polyamine
    Article Snippet: .. HUVECs (American Type Culture Collection (ATCC), PCS-100-013) were cultured in vascular cell basal medium supplemented with endothelial cell growth kit BBE (ATCC) and incubated at 37 °C with 5% CO 2 . .. Inhibitors, namely APCHA (0.5 mM; TCI chemicals), reserpine (10 μM; Cayman Chemical) or Baf-A1 (ref. ) (20 nM; Cayman Chemical), were added to mast cell culture 3 days before subsequent experiments.

    Incubation:

    Article Title: Mast cell extracellular granules are bioactive condensates assembled by heparin and polyamine.
    Article Snippet: .. HUVECs (American Type Culture Collection (ATCC), PCS-100-013) were cultured in vascular cell basal medium supplemented with endothelial cell growth kit BBE (ATCC) and incubated at 37 °C with 5% CO2. .. Inhibitors, namely APCHA 79 (0.5 mM; TCI chemicals), reserpine80 (10 μM; Cayman Chemical) or Baf-A1 (ref. 81) (20 nM; Cayman Chemical), were added to mast cell culture 3 days before subsequent experiments.

    Article Title: Mast cell extracellular granules are bioactive condensates assembled by heparin and polyamine
    Article Snippet: .. HUVECs (American Type Culture Collection (ATCC), PCS-100-013) were cultured in vascular cell basal medium supplemented with endothelial cell growth kit BBE (ATCC) and incubated at 37 °C with 5% CO 2 . .. Inhibitors, namely APCHA (0.5 mM; TCI chemicals), reserpine (10 μM; Cayman Chemical) or Baf-A1 (ref. ) (20 nM; Cayman Chemical), were added to mast cell culture 3 days before subsequent experiments.

    Modification:

    Article Title: STEAP3 alleviates inflammation and fibrosis via iron metabolism in ischemia/reperfusion-associated lung injury.
    Article Snippet: .. Human Umbilical Vein Endothelial Cells (HUVECs; American Type Culture Collection [ATCC], PCS-100-013) were cultured in Dulbecco's Modified Eagle's Medium (DMEM) with 10 % FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 ◦C with 5 % CO2. .. THP-1 cells (ATCC, TIB-202) were cultured in RPMI 1640 medium with 10 % FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 ◦C with 5 % CO2.



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    Regulatory role of HSPB1 in <t>endothelial</t> cell EndoMT (A) Western blot shows HSPB1 expression in <t>HUVECs</t> following lentiviral-mediated overexpression (LV-HSPB1) or knockdown (LV-HSPB1-RNAi); β-actin served as a loading control. (B) Quantification of HSPB1/β-actin ratio shows significant differences between groups. (C) Representative images of Transwell migration assays evaluating the effect of HSPB1 on TGF-β1–induced endothelial migration (scale bars, 100 μm). (D) Quantification of migrated cells per field. (E) Representative tube formation images showing the effect of HSPB1 modulation on TGF-β1–induced angiogenic activity (scale bars, 200 μm). (F–H) Quantitative analysis of tube formation parameters, including the number of branches (F), loops (G), and total tube length (H), measured using ImageJ software. Data are presented as mean ± SD ( n ≥ 6). Exact p values are indicated in the graphs. Statistical analyses were performed using one-way ANOVA followed by a Bonferroni post hoc test.
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    Image Search Results


    Regulatory role of HSPB1 in endothelial cell EndoMT (A) Western blot shows HSPB1 expression in HUVECs following lentiviral-mediated overexpression (LV-HSPB1) or knockdown (LV-HSPB1-RNAi); β-actin served as a loading control. (B) Quantification of HSPB1/β-actin ratio shows significant differences between groups. (C) Representative images of Transwell migration assays evaluating the effect of HSPB1 on TGF-β1–induced endothelial migration (scale bars, 100 μm). (D) Quantification of migrated cells per field. (E) Representative tube formation images showing the effect of HSPB1 modulation on TGF-β1–induced angiogenic activity (scale bars, 200 μm). (F–H) Quantitative analysis of tube formation parameters, including the number of branches (F), loops (G), and total tube length (H), measured using ImageJ software. Data are presented as mean ± SD ( n ≥ 6). Exact p values are indicated in the graphs. Statistical analyses were performed using one-way ANOVA followed by a Bonferroni post hoc test.

    Journal: iScience

    Article Title: Cardiomyocyte-derived HSPB1 regulates TGF-β1 maturation and inhibits endothelial-to-mesenchymal transition in myocardial fibrosis

    doi: 10.1016/j.isci.2026.115028

    Figure Lengend Snippet: Regulatory role of HSPB1 in endothelial cell EndoMT (A) Western blot shows HSPB1 expression in HUVECs following lentiviral-mediated overexpression (LV-HSPB1) or knockdown (LV-HSPB1-RNAi); β-actin served as a loading control. (B) Quantification of HSPB1/β-actin ratio shows significant differences between groups. (C) Representative images of Transwell migration assays evaluating the effect of HSPB1 on TGF-β1–induced endothelial migration (scale bars, 100 μm). (D) Quantification of migrated cells per field. (E) Representative tube formation images showing the effect of HSPB1 modulation on TGF-β1–induced angiogenic activity (scale bars, 200 μm). (F–H) Quantitative analysis of tube formation parameters, including the number of branches (F), loops (G), and total tube length (H), measured using ImageJ software. Data are presented as mean ± SD ( n ≥ 6). Exact p values are indicated in the graphs. Statistical analyses were performed using one-way ANOVA followed by a Bonferroni post hoc test.

    Article Snippet: Human umbilical vein endothelial cells (HUVECs) , ATCC , Primary cells, pooled donors.

    Techniques: Western Blot, Expressing, Over Expression, Knockdown, Control, Migration, Activity Assay, Software

    Effects of HSPB1 on signaling pathways and TGF-β secretion in HUVECs under hypoxic conditions (A and B) HUVECs were transfected with adenoviral vectors for HSPB1 overexpression (OE) or knockdown (KD) and cultured for 48 h before RNA extraction. Gene expression analysis was performed using RNA sequencing. Gene set enrichment analysis (GSEA) assessed the regulatory roles of HSPB1 in processes such as heart development, angiogenesis, and cell proliferation (A). Further analysis using Hallmark gene sets explored HSPB1 signaling pathway activation (B). (C–G) Following transfection, HUVECs were cultured for 24 h and subjected to hypoxic conditions (3% O 2 ) for 48 h. Western blot analysis of the indicated proteins was performed. (D) pSmad2/3/Smad2/3 ratio, (E) quantification of CD31 protein expression, (F) quantification of E-cadherin expression, (G) quantification of α-SMA expression, and (H) quantification of N-cadherin expression were measured relative to β-actin. (I) TGF-β levels were measured by ELISA in cell supernatants. Data are presented as mean ± SD ( n ≥ 6). Exact p values are indicated in the graphs. Statistical analyses were performed using one-way ANOVA followed by a Bonferroni post hoc test.

    Journal: iScience

    Article Title: Cardiomyocyte-derived HSPB1 regulates TGF-β1 maturation and inhibits endothelial-to-mesenchymal transition in myocardial fibrosis

    doi: 10.1016/j.isci.2026.115028

    Figure Lengend Snippet: Effects of HSPB1 on signaling pathways and TGF-β secretion in HUVECs under hypoxic conditions (A and B) HUVECs were transfected with adenoviral vectors for HSPB1 overexpression (OE) or knockdown (KD) and cultured for 48 h before RNA extraction. Gene expression analysis was performed using RNA sequencing. Gene set enrichment analysis (GSEA) assessed the regulatory roles of HSPB1 in processes such as heart development, angiogenesis, and cell proliferation (A). Further analysis using Hallmark gene sets explored HSPB1 signaling pathway activation (B). (C–G) Following transfection, HUVECs were cultured for 24 h and subjected to hypoxic conditions (3% O 2 ) for 48 h. Western blot analysis of the indicated proteins was performed. (D) pSmad2/3/Smad2/3 ratio, (E) quantification of CD31 protein expression, (F) quantification of E-cadherin expression, (G) quantification of α-SMA expression, and (H) quantification of N-cadherin expression were measured relative to β-actin. (I) TGF-β levels were measured by ELISA in cell supernatants. Data are presented as mean ± SD ( n ≥ 6). Exact p values are indicated in the graphs. Statistical analyses were performed using one-way ANOVA followed by a Bonferroni post hoc test.

    Article Snippet: Human umbilical vein endothelial cells (HUVECs) , ATCC , Primary cells, pooled donors.

    Techniques: Protein-Protein interactions, Transfection, Over Expression, Knockdown, Cell Culture, RNA Extraction, Gene Expression, RNA Sequencing, Activation Assay, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay